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In this video you can see RPE1 cells transfected with H2B-GFP plasmid (maximum intensity projection of 117 z planes). Courtesy of Tingsheng Liu, Mitosis Lab, Singapore. It shows the difference between LSM and the new LSM Plus processing. LSM Plus is a new linear Wiener filter deconvolution, that needs next to no interaction while still ensuring a reliable quantitative result. Regardless of whether you perform classic confocal or advanced multispectral imaging including NLO and NIR. Just as in th
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With Airyscan Joint Deconvolution (jDCV) super-resolution experiments benefit from an improved separation of single or multiple labels. The video shows a HeLa cell, 4× expanded and labelled with acetylated alpha tubulin (green). Courtesy of S. Zhang, Prof. Liou Yih-Cherng's lab, Singapore. Each of the 32 detector elements of Airyscan 2 has a slightly different view on the sample, providing additional spatial information that makes jDCV possible. This reduces the distance that can be resolved bet
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